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KMID : 0616120170480040298
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Korean Journal of Pharmacognosy
2017 Volume.48 No. 4 p.298 ~ p.303
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Anti-oxidative Effect of Epimedii Herba in Caenorhabditis elegans
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Kim Jun-Hyeong
An Chang-Wan
Kim Yeong-Jee
Noh Yun-Jeong
Kim Su-JIn
Hwang In-Hyun
Jeon Hoon
Cha Dong-Seok
Shin Tae-Yong
Kim Dae-Keun
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Abstract
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To know the anti-oxidative effect of Epimedii Herba (Berberidaceae), the methanol extract of this plant was investigated by using a Caenorhabditis elegans model system. The methanol extract of this plant showed relatively significant DPPH radical scavenging and superoxide quenching activities. The ethyl acetate soluble fraction of Epimedii Herba (EHE), which showed the most potent DPPH radical scavenging and superoxide quenching activities, was tested on its effects on superoxide dismutase (SOD), catalase, intracellular ROS, and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, in order to verify that regulation of stress-response genes is responsible for the increased stress tolerance of the EHE treated C. elegans, we checked SOD-3 expression using a transgenic strain. As a result, the EHE increased SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Besides, EHE-treated CF1553 worms showed higher SOD-3::GFP intensity than that of non-treated controls.
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KEYWORD
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Epimedii Herba, Caenorhabditis elegans, Anti-oxidative effect, SOD, ROS
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